Molecular Diagnostic techniques

The advent of polymerase chain reaction in the 1980s changed the face of diagnostics and remarkable innovation has been achieved since then. The progress in next-generation sequencing has revolutionized the field of molecular diagnostics and research.

The training has 3 modules:

The first module includes theory and practical sessions on different nucleic acid extraction methods, conventional PCR including agarose gel electrophoresis and nucleic acid quantitation techniques.

The second module consists of real-time PCR including both qualitative and quantitative PCR. The qualitative part includes uniplex and multiplex PCR, interpretation of results, and troubleshooting. The module also includes quality control in the PCR laboratory.

The final module includes the theory of DNA sequencing and next-generation sequencing methods. The practical aspect includes a demo of sequencing equipment and an analysis of DNA sequences.

Learning Objectives:

  • To provide practical knowledge to the participants on various molecular techniques used in the field of diagnostics and understand the theoretical aspects of Sanger sequencing as well as next-generation sequencing

Learning Outcome:

Upon completion of the course the participants should be able to:

  • Perform molecular techniques including nucleic acid extraction, conventional and real-time polymerase chain reaction
  • Understand the concept of nucleic acid sequencing
  • Recognize and troubleshoot problems in routine molecular diagnostic techniques

Course duration and Registration Details :

Date: 25 November 2024 to 29 November 2024

Last date for registration: 22 November 2024

Key Dates & Deadlines


Last date to Apply


Tentative Course Commencement Date